Composite

Part:BBa_K3757009:Design

Designed by: Erik Zimmer   Group: iGEM21_Tuebingen   (2021-09-28)


35S sGFP (S65T)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1575
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


Design Notes

Sequence optimized for plant codon-usage. Part can be used in Golden Gate Cloning of 3in1 binary vectors.


Source

GFP originates from Aequorea victoria, sGFP (S65T) mutant with improved fluorescent properties, obtained in lower-level Golden Gate vectors. 35S promoter and terminator originate from Cauliflower mosaic virus, obtained in lower-level Golden Gate vectors.

References